Difference between revisions of "Part:BBa K200003"

Revision as of 09:46, 1 September 2009

Rfal
Waal ligase is an enzyme responsible for the ligation of an O-antigen to the core oligosaccharide in the Gram-negative bacterium's outer membrane.

Unlike other exopolysaccharides, colanic acid does not naturally bind to the cell surface but rather forms a thick mesh between cells. While Waal Ligase usually links the O-antigen to the core oligosaccharide, in K-12 it links colanic acid to the core oligosaccharide. This is because E.coli K-12 does not produce O-antigen due to an insertion mutation.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 754
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

Requirements

Gram-negative bacterium
Colanic acid producing gene (rcsB)

References

function pmid=15838026
Function2 pmid=19019161

</biblio>

@@ Line 1: / Line 1: @@
 __NOTOC__
-<partinfo>BBa_K200003 short</partinfo>
+<partinfo>BBa_K200003 short</partinfo><br>
 Waal ligase is an enzyme responsible for the ligation of an O-antigen to the core oligosaccharide in the Gram-negative bacterium's outer membrane.
@@ Line 13: / Line 13: @@
 The Lipid A component of the LPS contains fatty acid chains which causes the LPS to dock into the outer membrane of the Gram-negative bacterium.
 <!-- -->
-<span class='h3bb'>Sequence and Features</span>
+<span class='h3bb'><b>Sequence and Features</b></span>
 <partinfo>BBa_K200003 SequenceAndFeatures</partinfo>
 ===Requirements===
 *Gram-negative bacterium
-*Colanic acid producing gene ([[BBa_K200000 | rcsB]])
+*Colanic acid producing gene ([[BBa_K200000|rcsB]])
 <!-- Uncomment this to enable Functional Parameter display