Difference between revisions of "Part:BBa K200003"
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===Requirements=== | ===Requirements=== | ||
*Gram-negative bacterium | *Gram-negative bacterium | ||
| − | *Colanic acid producing gene ([[BBa_K200000 rcsB]] | + | *Colanic acid producing gene ([[BBa_K200000 | rcsB]]) |
<!-- Uncomment this to enable Functional Parameter display | <!-- Uncomment this to enable Functional Parameter display | ||
Revision as of 09:44, 1 September 2009
Rfal Waal ligase is an enzyme responsible for the ligation of an O-antigen to the core oligosaccharide in the Gram-negative bacterium's outer membrane.
Unlike other exopolysaccharides, colanic acid does not naturally bind to the cell surface but rather forms a thick mesh between cells. While Waal Ligase usually links the O-antigen to the core oligosaccharide, in K-12 it links colanic acid to the core oligosaccharide. This is because E.coli K-12 does not produce O-antigen due to an insertion mutation.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 754
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Requirements
- Gram-negative bacterium
- Colanic acid producing gene ( rcsB)
References
<biblio>
- function pmid=15838026
- Function2 pmid=19019161
</biblio>