Difference between revisions of "Part:BBa K200007"
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<partinfo>BBa_K200007 short</partinfo>
 
<partinfo>BBa_K200007 short</partinfo>
  
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This is a BioBrick for cellulase production in E.coli. CelE is one of the three major proteins of the cellulosome of Clostridium cellulolyticum.
+
This is a BioBrick for cellulase production in E.coli. CelE is one of the three major proteins of the cellulosome of Clostridium cellulolyticum.<cite>1</cite>
  
 
Cellulase catalyses the reactions, from crystal to cellulose to cellobiose/cellotetrose and to glucose. The cellulose is protease resistant.
 
Cellulase catalyses the reactions, from crystal to cellulose to cellobiose/cellotetrose and to glucose. The cellulose is protease resistant.
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[http://www.ncbi.nlm.nih.gov/nuccore/125972525?report=genbank&log$=seqview&from=961597&to=964041 Sequence from NCBI]
 
[http://www.ncbi.nlm.nih.gov/nuccore/125972525?report=genbank&log$=seqview&from=961597&to=964041 Sequence from NCBI]
 +
<biblio>
 +
#1 pmid=168382
 +
</biblio>

Revision as of 08:37, 1 September 2009

Cellulase

This is a BioBrick for cellulase production in E.coli. CelE is one of the three major proteins of the cellulosome of Clostridium cellulolyticum.1

Cellulase catalyses the reactions, from crystal to cellulose to cellobiose/cellotetrose and to glucose. The cellulose is protease resistant.


As part of the Imperial 2009 iGEM E.ncapsulator project, E.coli was engineered to synthesise cellulase to a tunable threshold, and successfully coat and protect it through the stomach, until release in the small intestine.



Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 199
    Illegal PstI site found at 700
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 700
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 287
    Illegal BamHI site found at 814
    Illegal XhoI site found at 524
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 199
    Illegal PstI site found at 700
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 199
    Illegal PstI site found at 700
  • 1000
    COMPATIBLE WITH RFC[1000]


References

[http://www.ncbi.nlm.nih.gov/nuccore/125972525?report=genbank&log$=seqview&from=961597&to=964041 Sequence from NCBI] <biblio>

  1. 1 pmid=168382

</biblio>