Difference between revisions of "Part:BBa K200012"
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This lambda promoter contains 3 operator sites, as opposed to only 2 operator sites on the registry lambda promoter (BBa_R0051) <br> | This lambda promoter contains 3 operator sites, as opposed to only 2 operator sites on the registry lambda promoter (BBa_R0051) <br> | ||
| − | It has been shown through lysis experiments that this lambda promoter can be stringently repressed by the cI protein at temperatures up to 30°C. At temperatures higher than 30°C, the cI repressor is progressively thermally denatured and no longer able to bind to the promoter operator regions. Therefore, promoter activity is induced. <br> | + | It has been shown through lysis experiments <cite>pL1</cite> that this lambda promoter can be stringently repressed by the cI protein at temperatures up to 30°C. At temperatures higher than 30°C, the cI repressor is progressively thermally denatured and no longer able to bind to the promoter operator regions. Therefore, promoter activity is induced. <br> |
The system of the lambda promoter- temperature sensitive cI protein provides an effective way to regulate gene expression using temperature. <br> | The system of the lambda promoter- temperature sensitive cI protein provides an effective way to regulate gene expression using temperature. <br> | ||
| + | |||
| + | |||
| + | ===References=== | ||
| + | <biblio> | ||
| + | pL1 pmid=10227165 | ||
| + | </biblio> | ||
Revision as of 20:44, 31 August 2009
lambda promoter (cIts responsive)
This is different from the common lambda promoter in that it is able to be repressed by the temperature sensitive cI protein (BBa_K200011).
This lambda promoter contains 3 operator sites, as opposed to only 2 operator sites on the registry lambda promoter (BBa_R0051)
It has been shown through lysis experiments pL1 that this lambda promoter can be stringently repressed by the cI protein at temperatures up to 30°C. At temperatures higher than 30°C, the cI repressor is progressively thermally denatured and no longer able to bind to the promoter operator regions. Therefore, promoter activity is induced.
The system of the lambda promoter- temperature sensitive cI protein provides an effective way to regulate gene expression using temperature.
References
<biblio> pL1 pmid=10227165 </biblio>