Difference between revisions of "Part:BBa K200009"
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Dam Sensitive Restriction Enzyme.
 
Dam Sensitive Restriction Enzyme.
 
The recognition sequence of this restriction enzyme is GATC, and this enzyme is a 4 base cutting enzyme leaving a 4 base overhang.
 
The recognition sequence of this restriction enzyme is GATC, and this enzyme is a 4 base cutting enzyme leaving a 4 base overhang.
 +
  
 
<br>
 
<br>
 
The Cutting Site is as shown:
 
The Cutting Site is as shown:
 
<br>
 
<br>
−
NGATCN  ->  N GATCN <br>
+
NGATCN  ->  N / GATCN <br>
−
NCTAGN  ->  NCTAG N   
+
NCTAGN  ->  NCTAG / N   
 +
 
 +
 
 +
As part of the Imperial 2009 iGEM E.ncapsulator project, DpnII was used to cut up the genetic material within the cell, completing the cell death module.
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 +
 
 +
 
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 14:19, 28 August 2009

DpnII Composite

Dam Sensitive Restriction Enzyme. The recognition sequence of this restriction enzyme is GATC, and this enzyme is a 4 base cutting enzyme leaving a 4 base overhang.



The Cutting Site is as shown:
NGATCN -> N / GATCN
NCTAGN -> NCTAG / N


As part of the Imperial 2009 iGEM E.ncapsulator project, DpnII was used to cut up the genetic material within the cell, completing the cell death module.



Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]