Difference between revisions of "Part:BBa K200005:Design"
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Revision as of 11:59, 28 August 2009
OtsA: Part 1 of 2 for trehalose producing enzymes.
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 383
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
PCR from Escherichia Coli BL21(DE3)
Forward: GCTCTAGATGAGTCGTTTAGTCGTAG
Recomended Temperatures for PCR : 52.4C(without overhang) and 63.2(with overhang)
Backward: GGACTAGTACTACGCAAGCTTTGGAAAG
Recomended Temperatures for PCR : 54.5C(without overhang) and 65.1(with overhang)
Source
Genes involved found in literature, and isolated by PCR of Escherichia coli BL21(DE3)