Difference between revisions of "Part:BBa K200005:Design"

(→‎Design Notes)
(→‎Design Notes)
Line 8: Line 8:
 
Used to resist dessication
 
Used to resist dessication
  
−
Forward: <b>GCTCTAG</b>ATGAGTCGTTTAGTCGTAG      52.4C(without) and 63.2(with)
+
Forward: <b>GCTCTAG</b>ATGAGTCGTTTAGTCGTAG      <br>
−
Backward: <b>GGACTAGTA</b>CTACGCAAGCTTTGGAAAG      54.5C(without) and 65.1(with)
+
Recomended Temperatures for PCR : 52.4C(without overhang) and 63.2(with overhang)<br><br>
 +
Backward: <b>GGACTAGTA</b>CTACGCAAGCTTTGGAAAG       
 +
Recomended Temperatures for PCR : 54.5C(without overhang) and 65.1(with overhang)
  
 
===Source===
 
===Source===

Revision as of 11:38, 28 August 2009

OtsA: Part 1 of 2 for trehalose producing enzymes.


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 383
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Used to resist dessication

Forward: GCTCTAGATGAGTCGTTTAGTCGTAG
Recomended Temperatures for PCR : 52.4C(without overhang) and 63.2(with overhang)

Backward: GGACTAGTACTACGCAAGCTTTGGAAAG Recomended Temperatures for PCR : 54.5C(without overhang) and 65.1(with overhang)

Source

Escherichia coli. Sourced from ecoliwiki

References