Difference between revisions of "Part:BBa K200008"
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[http://en.wikipedia.org/wiki/Phenylalanine_hydroxylase Phenylalanine hydroxylase (PAH)] is the enzyme that determines the rate-limiting step in phenylalanine catabolism. Deficiency of this enzyme activity results in the autosomal recessive disorder phenylketonuria.
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[http://en.wikipedia.org/wiki/Phenylalanine_hydroxylase Phenylalanine hydroxylase (PAH)] is the enzyme that determines the rate-limiting step in phenylalanine catabolism. Deficiency of this enzyme activity results in the autosomal recessive disorder phenylketonuria.<cite>#PAH1</cite>
 
<br>
 
<br>
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As part of the Imperial 2009 iGEM E.ncapsulator project, E.coli was engineered to synthesise PAH to a tunable threshold, and successfully  coat and protect it through the stomach, until release in the small intestine.  
 
As part of the Imperial 2009 iGEM E.ncapsulator project, E.coli was engineered to synthesise PAH to a tunable threshold, and successfully  coat and protect it through the stomach, until release in the small intestine.  
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=References=
 
=References=
 
[http://www.ncbi.nlm.nih.gov/nuccore/NM_000277.1?report=fasta Sequence from NCBI]
 
[http://www.ncbi.nlm.nih.gov/nuccore/NM_000277.1?report=fasta Sequence from NCBI]
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<biblio>#PAH1 pmid = 2014036 </biblio>

Revision as of 10:36, 28 August 2009

Phenylalanine hydroxylase


[http://en.wikipedia.org/wiki/Phenylalanine_hydroxylase Phenylalanine hydroxylase (PAH)] is the enzyme that determines the rate-limiting step in phenylalanine catabolism. Deficiency of this enzyme activity results in the autosomal recessive disorder phenylketonuria.#PAH1

As part of the Imperial 2009 iGEM E.ncapsulator project, E.coli was engineered to synthesise PAH to a tunable threshold, and successfully coat and protect it through the stomach, until release in the small intestine.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 287
    Illegal BamHI site found at 814
    Illegal XhoI site found at 524
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]



References

[http://www.ncbi.nlm.nih.gov/nuccore/NM_000277.1?report=fasta Sequence from NCBI] <biblio>#PAH1 pmid = 2014036 </biblio>