Difference between revisions of "Part:BBa K4140007"
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==Part Description== | ==Part Description== | ||
| − | KP-SP is a secreted signal peptide at the N-terminus of the reporter protein that enables it's secretion extracellularly. this peptide comes from E.Coli as well as actinomycetes and can be secreted under normal instances | + | KP-SP is a secreted signal peptide at the N-terminus of the reporter protein that enables it's secretion extracellularly with high efficiency which s indicates the versatility of this signal peptide and its significant potential in heterologous protein expression. this peptide comes from E.Coli as well as actinomycetes and can be secreted under normal instances |
==Usage== | ==Usage== | ||
Revision as of 15:57, 30 September 2022
kp-sp
Part Description
KP-SP is a secreted signal peptide at the N-terminus of the reporter protein that enables it's secretion extracellularly with high efficiency which s indicates the versatility of this signal peptide and its significant potential in heterologous protein expression. this peptide comes from E.Coli as well as actinomycetes and can be secreted under normal instances
Usage
To transmit the response of our circuit to high levels of phenylalanine from intracellular to extracellular we linked Kp Sp signal at the N-terminus of the PAH for transmitting it extracellularly to hydroxylate excess phenylalanine into tyrosine.
Literature Characterization
References
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]