Difference between revisions of "Part:BBa K4488008:Design"

 
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__NOTOC__
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<partinfo>BBa_K4488008 short</partinfo>
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<partinfo>BBa_K4488008 SequenceAndFeatures</partinfo>
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===Design Notes===
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The construct has a constitutive RBS (BBa_B0034). A 6bp cloning scar (“accgcc”) was also inserted between the fuGFP and CBDclos, similar to BBa_K1321342, to act as a small linker. NdeI recognition site was removed from CBDclos sequence by changing the codon for threonine (“aca” to “acT”). Many base pairs were removed to avoid an inverted repeat and the codon usage at a GC rich region was changed.
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===Source===
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The design of the part was based on the sfGFP-CBD fusion protein (BBa_K1321342) developed by the 2014 Imperial team Aqualose. The fuGFP sequence can be found here (BBa_K3814004).
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===References===

Revision as of 08:51, 30 September 2022

Fusion of free-use GFP with CBDcex (cellulose-binding domain) at the C-terminal end


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 163
  • 1000
    COMPATIBLE WITH RFC[1000]

Design Notes

The construct has a constitutive RBS (BBa_B0034). A 6bp cloning scar (“accgcc”) was also inserted between the fuGFP and CBDclos, similar to BBa_K1321342, to act as a small linker. NdeI recognition site was removed from CBDclos sequence by changing the codon for threonine (“aca” to “acT”). Many base pairs were removed to avoid an inverted repeat and the codon usage at a GC rich region was changed.

Source

The design of the part was based on the sfGFP-CBD fusion protein (BBa_K1321342) developed by the 2014 Imperial team Aqualose. The fuGFP sequence can be found here (BBa_K3814004).

References