Difference between revisions of "Part:BBa K4488007:Design"
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__NOTOC__ | __NOTOC__ | ||
| − | <partinfo> | + | <partinfo>BBa_K4488007 short</partinfo> |
| − | <partinfo> | + | <partinfo>BBa_K4488007 SequenceAndFeatures</partinfo> |
===Design Notes=== | ===Design Notes=== | ||
| − | + | The construct has a constitutive RBS (BBa_B0034). 6bp cloning scar (“accgcc”) was also inserted between the fuGFP and CBDclos, similar to BBa_K1321341, to act as a small linker. NdeI recognition site was removed from CBDclos sequence by changing the codon for threonine (“aca” to “acT”). A few base pairs were removed to avoid an inverted repeat and the codon usage of ≈200bp at a GC rich region was changed. | |
===Source=== | ===Source=== | ||
| − | + | The design of the part was based on the sfGFP-CBD fusion protein (BBa_K1321341) developed by the 2014 Imperial team Aqualose. The fuGFP sequence can be found here (BBa_K3814004). | |
===References=== | ===References=== | ||
Revision as of 08:48, 30 September 2022
Fusion of free-use GFP with CBDclos (cellulose-binding domain) at the C-terminal end
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 163
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
The construct has a constitutive RBS (BBa_B0034). 6bp cloning scar (“accgcc”) was also inserted between the fuGFP and CBDclos, similar to BBa_K1321341, to act as a small linker. NdeI recognition site was removed from CBDclos sequence by changing the codon for threonine (“aca” to “acT”). A few base pairs were removed to avoid an inverted repeat and the codon usage of ≈200bp at a GC rich region was changed.
Source
The design of the part was based on the sfGFP-CBD fusion protein (BBa_K1321341) developed by the 2014 Imperial team Aqualose. The fuGFP sequence can be found here (BBa_K3814004).