Difference between revisions of "Part:BBa K4408008"
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This part is responsible for expressing adhE2 protein. The expression is controlled by a Pthl promoter with an ribosome binding site (RBS) and a Cpa fdx terminator. It consists of BBa_K3443002(Pthl), BBa_K4408001(RBS for adhE2), BBa_K1462060(adhE2), and BBa_K3585002(Cpa fdx terminator). In our program, we use this device to construct the synthesis pathways of butanol in Clostridium tyrobutyricum. By introducing adhE2，butyryl-CoA is converted to butyl aldehyde and further transformed to butanol. Pthl starts the transcription and Cpa fdx terminator ends the transcription. | This part is responsible for expressing adhE2 protein. The expression is controlled by a Pthl promoter with an ribosome binding site (RBS) and a Cpa fdx terminator. It consists of BBa_K3443002(Pthl), BBa_K4408001(RBS for adhE2), BBa_K1462060(adhE2), and BBa_K3585002(Cpa fdx terminator). In our program, we use this device to construct the synthesis pathways of butanol in Clostridium tyrobutyricum. By introducing adhE2，butyryl-CoA is converted to butyl aldehyde and further transformed to butanol. Pthl starts the transcription and Cpa fdx terminator ends the transcription. | ||
| − | Add more about the biology of this part here | + | <!-- Add more about the biology of this part here |
===Usage and Biology=== | ===Usage and Biology=== | ||
Revision as of 08:24, 30 September 2022
Pthl-adhE2, Expression of adhE2 with Pthl promoter
This part is responsible for expressing adhE2 protein. The expression is controlled by a Pthl promoter with an ribosome binding site (RBS) and a Cpa fdx terminator. It consists of BBa_K3443002(Pthl), BBa_K4408001(RBS for adhE2), BBa_K1462060(adhE2), and BBa_K3585002(Cpa fdx terminator). In our program, we use this device to construct the synthesis pathways of butanol in Clostridium tyrobutyricum. By introducing adhE2，butyryl-CoA is converted to butyl aldehyde and further transformed to butanol. Pthl starts the transcription and Cpa fdx terminator ends the transcription.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]