Difference between revisions of "Part:BBa K4212009"

Revision as of 21:43, 29 September 2022

GerAK_chimera

Synthetic operon designed with GerAA, GerKB and GerAC coding sequences from the B. subtilis genome. Designed to be transformed in a B. subtilis knockout strain so as to produce a germinant receptor that contains a binding site for glucose, and when activated triggers an orthogonal signalling cascade resulting in germination. The GerAA sequence features a C-terminus fusion to a GFPmut3b coding sequence. This is to enable visualisation of the chimeric receptor on the surface of the spore via fluorescence microscopy.

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal EcoRI site found at 2384
Illegal EcoRI site found at 4339
Illegal PstI site found at 533
Illegal PstI site found at 4290
12
INCOMPATIBLE WITH RFC[12]
Illegal EcoRI site found at 2384
Illegal EcoRI site found at 4339
Illegal NheI site found at 4
Illegal PstI site found at 533
Illegal PstI site found at 4290
21
INCOMPATIBLE WITH RFC[21]
Illegal EcoRI site found at 2384
Illegal EcoRI site found at 4339
Illegal BglII site found at 3871
Illegal XhoI site found at 640
23
INCOMPATIBLE WITH RFC[23]
Illegal EcoRI site found at 2384
Illegal EcoRI site found at 4339
Illegal PstI site found at 533
Illegal PstI site found at 4290
25
INCOMPATIBLE WITH RFC[25]
Illegal EcoRI site found at 2384
Illegal EcoRI site found at 4339
Illegal PstI site found at 533
Illegal PstI site found at 4290
1000
INCOMPATIBLE WITH RFC[1000]
Illegal SapI site found at 3526

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K4212009 short</partinfo>
-Synthetic operon designed with GerAA, GerKB and GerAC coding sequences from the B. subtilis genome. Designed to be transformed in a B. subtilis knockout strain so as to produce a germinant receptor that contains a binding site for glucose, and when activated triggers an orthogonal signalling cascade resulting in germination.
+Synthetic operon designed with GerAA, GerKB and GerAC coding sequences from the B. subtilis genome. Designed to be transformed in a B. subtilis knockout strain so as to produce a germinant receptor that contains a binding site for glucose, and when activated triggers an orthogonal signalling cascade resulting in germination. The GerAA sequence features a C-terminus fusion to a GFPmut3b coding sequence. This is to enable visualisation of the chimeric receptor on the surface of the spore via fluorescence microscopy.
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