Difference between revisions of "Part:BBa K4447004"
Line 3: | Line 3: | ||
<partinfo>BBa_K4447004 short</partinfo> | <partinfo>BBa_K4447004 short</partinfo> | ||
− | FRET-based sensor system for the detection of erythromycin that consists of erythromycin C-12 hydroxylase [https://parts.igem.org/Part:BBa_K4447001 (BBa_K4447001)],an enzyme that catalyzes the oxidation of erythromycin, flanked by two fluorescent proteins: ECFP [https://parts.igem.org/Part:BBa_K1159302 (BBa_K1159302)]as an energy donor and Venus [https://parts.igem.org/Part:BBa_1907000 (BBa_1907000)]as an energy acceptor. | + | FRET-based sensor system for the detection of erythromycin that consists of erythromycin C-12 hydroxylase [https://parts.igem.org/Part:BBa_K4447001(BBa_K4447001)],an enzyme that catalyzes the oxidation of erythromycin, flanked by two fluorescent proteins: ECFP [https://parts.igem.org/Part:BBa_K1159302(BBa_K1159302)]as an energy donor and Venus [https://parts.igem.org/Part:BBa_1907000(BBa_1907000)]as an energy acceptor. |
<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here |
Revision as of 19:07, 29 September 2022
FRET-based system for the detection of erythromycin
FRET-based sensor system for the detection of erythromycin that consists of erythromycin C-12 hydroxylase [1],an enzyme that catalyzes the oxidation of erythromycin, flanked by two fluorescent proteins: ECFP [2]as an energy donor and Venus [3]as an energy acceptor.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 1913
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 2562