Difference between revisions of "Part:BBa K257000"
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Inducible promoter controled by the FecARI pathway. | Inducible promoter controled by the FecARI pathway. | ||
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===Usage and Biology=== | ===Usage and Biology=== | ||
The fec transduction pathway is unique because it allows a signal to be passed from the extracellular milieu down to the cytoplasm, thus being transmitted through both the outer and inner membrane. Briefly, the outermembrane protein FecA binds ferric citrate, change its conformation to interact with FecR which is an transmembrane protein located in the inner membrane. Then FecR is able to activate FecI an alternative sigma factor specific to Pfec. | The fec transduction pathway is unique because it allows a signal to be passed from the extracellular milieu down to the cytoplasm, thus being transmitted through both the outer and inner membrane. Briefly, the outermembrane protein FecA binds ferric citrate, change its conformation to interact with FecR which is an transmembrane protein located in the inner membrane. Then FecR is able to activate FecI an alternative sigma factor specific to Pfec. | ||
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In some variant of the system, the activated FecR homologues would repress FecI activity instead of activating it. | In some variant of the system, the activated FecR homologues would repress FecI activity instead of activating it. | ||
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For more information, see: Gene regulation by transmembrane signaling. Volkmar Braun et al., 2006 | For more information, see: Gene regulation by transmembrane signaling. Volkmar Braun et al., 2006 | ||
Revision as of 15:54, 20 August 2009
fec promoter (Pfec) - inducible through the Fec transduction pathway (ferric citrate)
Inducible promoter controled by the FecARI pathway.
Usage and Biology
The fec transduction pathway is unique because it allows a signal to be passed from the extracellular milieu down to the cytoplasm, thus being transmitted through both the outer and inner membrane. Briefly, the outermembrane protein FecA binds ferric citrate, change its conformation to interact with FecR which is an transmembrane protein located in the inner membrane. Then FecR is able to activate FecI an alternative sigma factor specific to Pfec.
In some variant of the system, the activated FecR homologues would repress FecI activity instead of activating it.
For more information, see: Gene regulation by transmembrane signaling. Volkmar Braun et al., 2006
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]