Difference between revisions of "Part:BBa K4192110"

Revision as of 17:18, 29 September 2022

cdg-gacA

RBS-cdg-RBS-gacA is composed of cdg gene (BBa_K4192010), gacA gene (BBa_4192011) and strong RBS sequence of Pseudomonas fluorescens (BBa_K4192000). cdg and gacA encode regulatory factors DGC and GacA in Pseudomonas fluorescens, which can ncrease the expression of extracellular polysaccharide (EPS) and then increase the production of biofilm. This part has been connected to the downstream of Plac and transferred into E. coli and Pseudomonas fluorescens 2P24 for testing. The data are as follows: The results showed that it was difficult to express in E. coli, but it had a good expression effect in Pseudomonas fluorescens 2P24.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 268
Illegal BamHI site found at 259
Illegal XhoI site found at 357
Illegal XhoI site found at 801
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 192
Illegal NgoMIV site found at 1289
Illegal AgeI site found at 759
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 210
Illegal BsaI.rc site found at 777

@@ Line 5: / Line 5: @@
 RBS-cdg-RBS-gacA is composed of cdg gene (BBa_K4192010), gacA gene (BBa_4192011) and strong RBS sequence of Pseudomonas fluorescens (BBa_K4192000).
 cdg and gacA encode regulatory factors DGC and GacA in Pseudomonas fluorescens, which can ncrease the expression of extracellular polysaccharide (EPS) and then increase the production of biofilm.
-This part has been tested to be effective in Pseudomonas fluorescens 2P24.
+This part has been connected to the downstream of Plac and transferred into E. coli and Pseudomonas fluorescens 2P24 for testing. The data are as follows:
+The results showed that it was difficult to express in E. coli, but it had a good expression effect in Pseudomonas fluorescens 2P24.
 <!-- Add more about the biology of this part here