Difference between revisions of "Part:BBa K4192110"
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<partinfo>BBa_K4192110 short</partinfo> | <partinfo>BBa_K4192110 short</partinfo> | ||
− | RBS- | + | RBS-cdg-RBS-gacA is composed of cdg gene (BBa_K4192010), gacA gene (BBa_4192011) and strong RBS sequence of Pseudomonas fluorescens (BBa_K4192000). |
cdg and gacA encode regulatory factors DGC and GacA in Pseudomonas fluorescens, which can ncrease the expression of extracellular polysaccharide (EPS) and then increase the production of biofilm. | cdg and gacA encode regulatory factors DGC and GacA in Pseudomonas fluorescens, which can ncrease the expression of extracellular polysaccharide (EPS) and then increase the production of biofilm. | ||
This part has been tested to be effective in Pseudomonas fluorescens 2P24. | This part has been tested to be effective in Pseudomonas fluorescens 2P24. |
Revision as of 17:14, 29 September 2022
cdg-gacA
RBS-cdg-RBS-gacA is composed of cdg gene (BBa_K4192010), gacA gene (BBa_4192011) and strong RBS sequence of Pseudomonas fluorescens (BBa_K4192000). cdg and gacA encode regulatory factors DGC and GacA in Pseudomonas fluorescens, which can ncrease the expression of extracellular polysaccharide (EPS) and then increase the production of biofilm. This part has been tested to be effective in Pseudomonas fluorescens 2P24.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 268
Illegal BamHI site found at 259
Illegal XhoI site found at 357
Illegal XhoI site found at 801 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 192
Illegal NgoMIV site found at 1289
Illegal AgeI site found at 759 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 210
Illegal BsaI.rc site found at 777