Difference between revisions of "Part:BBa K190019:Design"
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fMT is a metallothionein, binding Arsenite(III) and Arsenate(V), it has higher affinity for As(III). Overexpression of fMT can be used to accumulate Arsenic without toxification of the cells.  
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fMT is a metallothionein, binding Arsenite(III) and Arsenate(V), it has higher affinity for As(III).  
  
 
<partinfo>BBa_K190019 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K190019 SequenceAndFeatures</partinfo>

Revision as of 15:51, 19 August 2009

fMT


fMT is a metallothionein, binding Arsenite(III) and Arsenate(V), it has higher affinity for As(III).


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 38
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 127
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Add BioBrick prefix and suffix, also a RBS (B0034) will be added (not in sequence yet)

Source

fMT was cloned from pMAL-MT (kindly provided by Wilfred Chen, University of California), the origin of the gene is the genome of Fucus vesiculosus.

References

Highly Selective and Rapid Arsenic Removal by Metabolically Engineered Escherichia coli Cells Expressing Fucus vesiculosus Metallothionein. Singh et al. App and Env Microbiology, May 2008, p. 2924–2927