Difference between revisions of "Part:BBa K190019:Design"
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fMT is a metallothionein, binding Arsenite(III) and Arsenate(V), it has higher affinity for As(III). Overexpression of fMT can be used to accumulate Arsenic without toxification of the cells. | fMT is a metallothionein, binding Arsenite(III) and Arsenate(V), it has higher affinity for As(III). Overexpression of fMT can be used to accumulate Arsenic without toxification of the cells. | ||
Revision as of 14:49, 19 August 2009
fMT
fMT is a metallothionein, binding Arsenite(III) and Arsenate(V), it has higher affinity for As(III). Overexpression of fMT can be used to accumulate Arsenic without toxification of the cells.
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 38
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 127
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Add BioBrick prefix and suffix, also a RBS (B0034) will be added (not in sequence yet)
Source
fMT was cloned from pMAL-MT (kindly provided by Wilfred Chen, University of California), the origin of the gene is the genome of Fucus vesiculosus.
References
Highly Selective and Rapid Arsenic Removal by Metabolically Engineered Escherichia coli Cells Expressing Fucus vesiculosus Metallothionein. Singh et al. App and Env Microbiology, May 2008, p. 2924–2927