Difference between revisions of "Part:BBa K4140017"

Revision as of 10:05, 29 September 2022

CMV promoter

Part Description

CMV (cytomegalovirus) promoter is a strong constitutive promoter commonly used in mammalian expression systems to produce high levels of expression, However CMV promoter is on of the most variable types of promoter as it is highly variable between different cell types such as being strong in some cell types and being much weaker in other types

Usage

We used a mammalian expression systems to regulate the expression of Cas12g which have a remarkable role on tuning and controlling our circuit activity and the levels of PAH to be constant within normal level to avoid

Literature Characterization

Figure 1.Substrate RNA cleavage assay using wild-type and mutant target RNAs.

References

Sequence and Features