Difference between revisions of "Part:BBa K4140007"

Revision as of 08:35, 29 September 2022

kp-sp

Part Description

KP-SP is a secreted signal peptide at the N-terminus of the reporter protein that enables it's secretion extracellularly. this peptide comes from E.Coli as well as actinomycetes and can be secreted under normal instances

Usage

To transmit the response of our circuit to high levels of phenylalanine from intracellular to extracellular we linked Kp Sp signal at the N-terminus of the PAH for transmitting it extracellularly to hydroxylate excess phenylalanine into tyrosine.

Literature Characterization

Figure 1.Substrate RNA cleavage assay using wild-type and mutant target RNAs.

References

Sequence and Features