Difference between revisions of "Part:BBa K239008:Design"
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===References=== | ===References=== | ||
| + | Brown, L. et al. (2002) DksA affects ppGpp induction of RpoS at a | ||
| + | translational level. J. Bacteriol. 184, 4455–4465 | ||
Latest revision as of 12:26, 17 August 2009
RBS from RpoS
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Detect and remove possible restriction sites: XbaI, EcoRI, PstI, SpeI, AgeI, BamHI, BglII, NogMIV, NotI and XhoI. No restriction site had to be removed.
Source
Genomic sequence from biocyc.org database. Escherichia coli K-12 substr. MG1655 Native unmodified RBS for RpoS, but RpoS transcription can be initiated at more than one position. Hence other native RBS are also possible.
References
Brown, L. et al. (2002) DksA affects ppGpp induction of RpoS at a translational level. J. Bacteriol. 184, 4455–4465