Difference between revisions of "Part:BBa K4447003:Design"

 
 
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__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K4447003 short</partinfo>
 
<partinfo>BBa_K4447003 short</partinfo>
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<div style="text-align:justify;">
 
<partinfo>BBa_K4447003 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K4447003 SequenceAndFeatures</partinfo>
  
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===Design Notes===
 
===Design Notes===
 
A gly-gly-ser spacer, and a polyhistidine-tag were added before stop codon for protein purification. NcoI and XhoI restriction sites were added in 5' and 3' ends for protein overexpression in pBAD/Myc-His plasmids.
 
A gly-gly-ser spacer, and a polyhistidine-tag were added before stop codon for protein purification. NcoI and XhoI restriction sites were added in 5' and 3' ends for protein overexpression in pBAD/Myc-His plasmids.
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===Source===
 
===Source===
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The original sequence was reported by Ishikawa <i>et al.</i> (2004). Sequence can be found on GenBank (NG_052045.1).
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The original sequence was reported by Hoshino et al. (2010). Sequence can be found on GenBank (NG_052045.1).
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===References===
 
===References===
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[1]. Ishikawa, J., Yamashita, A., Mikami, Y., Hoshino, Y., Kurita, H., Hotta, K., Shiba, T., & Hattori, M. (2004). The complete genomic sequence of <i>Nocardia farcinica</i> IFM 10152. <i>Proceedings of the National Academy of Sciences of the United States of America, 101</i>(41), 14925–14930. https://doi.org/10.1073/pnas.0406410101

Latest revision as of 04:06, 29 September 2022


RifMo coding sequence


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 1225
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 1456
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

A gly-gly-ser spacer, and a polyhistidine-tag were added before stop codon for protein purification. NcoI and XhoI restriction sites were added in 5' and 3' ends for protein overexpression in pBAD/Myc-His plasmids.

Source

The original sequence was reported by Ishikawa et al. (2004). Sequence can be found on GenBank (NG_052045.1).

References

[1]. Ishikawa, J., Yamashita, A., Mikami, Y., Hoshino, Y., Kurita, H., Hotta, K., Shiba, T., & Hattori, M. (2004). The complete genomic sequence of Nocardia farcinica IFM 10152. Proceedings of the National Academy of Sciences of the United States of America, 101(41), 14925–14930. https://doi.org/10.1073/pnas.0406410101