Difference between revisions of "Part:BBa K4194008"
 
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===Usage and Biology===
 
===Usage and Biology===
 
==ssrA-LVA tag==
 
==ssrA-LVA tag==
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[[File:Mechanism of ssrA-LVA tag.png|300px|thumb|left|alt text]]LVA tag is commonly found attached to repressors in various gene networks (e.g. oscillators). The tag encodes peptide sequence AANDENYALVA and is recognized by ClpA and ClpX unfoldases and ClpX mediator SspB. ClpA and ClpX then form a proteosome-like complex with ClpP protease and the protein is degraded.
+
[[File:Mechanism of ssrA-LVA tag.png|300px|thumb|left|In E. coli [wild-type (sspB+ clpX+)], the adaptor sspB binds ssrAtagged substrates to ClpXP proteases, resulting in a modest increase in their degradation rate.]]LVA tag is commonly found attached to repressors in various gene networks (e.g. oscillators). The tag encodes peptide sequence AANDENYALVA and is recognized by ClpA and ClpX unfoldases and ClpX mediator SspB. ClpA and ClpX then form a proteosome-like complex with ClpP protease and the protein is degraded.
 
<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
 
<partinfo>BBa_K4194008 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K4194008 SequenceAndFeatures</partinfo>
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===Functional Parameters===
 
===Functional Parameters===
 
<partinfo>BBa_K4194008 parameters</partinfo>
 
<partinfo>BBa_K4194008 parameters</partinfo>
−
[[File:表达了mRFP1的红色菌液.jpeg|200px|thumb|left|alt text]]
+
[[File:表达了mRFP1的红色菌液.jpeg|200px|thumb|left|The bacteria solution is red because of the expression of mRFP1.]]

Latest revision as of 02:16, 29 September 2022


RFP from Discosoma striata (coral) with LVA-ssrA degradation tag

Mutant RFP from Discosoma striata (coral) with added LVA-ssrA degradation tag. The tag increases RFP turn-over rate, thus providing better temporal resolution of red fluorescence. In the same time, maximal fluorescence amplitudes will be lower as newly formed protein is degraded as soon as it is formed.


Usage and Biology

ssrA-LVA tag

In E. coli [wild-type (sspB+ clpX+)], the adaptor sspB binds ssrAtagged substrates to ClpXP proteases, resulting in a modest increase in their degradation rate.
LVA tag is commonly found attached to repressors in various gene networks (e.g. oscillators). The tag encodes peptide sequence AANDENYALVA and is recognized by ClpA and ClpX unfoldases and ClpX mediator SspB. ClpA and ClpX then form a proteosome-like complex with ClpP protease and the protein is degraded.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 673
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 555
  • 1000
    COMPATIBLE WITH RFC[1000]


Functional Parameters

colorred
emission607
excitation584
tagssrA-LVA tag
The bacteria solution is red because of the expression of mRFP1.