Difference between revisions of "Part:BBa K4387000"
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<partinfo>BBa_K4387000 short</partinfo>
 
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PnorV_beta is an optimized nitric oxide sensitive promoter. The promoter is coupled to sfGFP and NorR. Two corresponding transcription factors IHF1 and IHF2 were removed from the promoter PnorV to exhibit good sensitivity and dosage response at a low range of inducer DETA/NO, the used NO source in the experiment, activating the downstream genes' transcription.
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PnorV_beta is an optimized nitric oxide sensitive promoter. The promoter is coupled to sfGFP and NorR. Two corresponding integration host factors IHF1 and IHF2 binding sites were removed from the promoter PnorV to exhibit good sensitivity and dosage response at a low range of inducer DETA/NO, the used NO source in the experiment, activating the downstream genes' transcription.
  
 
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Revision as of 20:58, 28 September 2022


Nitric Oxide Sensing Promoter pNorVβ

PnorV_beta is an optimized nitric oxide sensitive promoter. The promoter is coupled to sfGFP and NorR. Two corresponding integration host factors IHF1 and IHF2 binding sites were removed from the promoter PnorV to exhibit good sensitivity and dosage response at a low range of inducer DETA/NO, the used NO source in the experiment, activating the downstream genes' transcription.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]