Difference between revisions of "Part:BBa K4387000"

 
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<partinfo>BBa_K4387000 short</partinfo>
 
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PNorV_beta is an optimized nitric oxide sensitive promoter. The promoter is coupled to a positive feedback loop with sfGFP and NorR, the corresponding transcription factor. IHF was removed from the promoter pNorV to exhibit good sensitivity and dosage response at a low range of inducer DETA/NO, the used NO source in the experiment, activating the downstream genes' transcription.
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PnorV_beta is an optimized nitric oxide sensitive promoter. The promoter is coupled to a positive feedback loop with sfGFP and norR, the corresponding transcription factor. IHF was removed from the promoter PnorV to exhibit good sensitivity and dosage response at a low range of inducer DETA/NO, the used NO source in the experiment, activating the downstream genes' transcription.
  
 
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Revision as of 19:19, 28 September 2022


Nitric Oxide Sensing Promoter pNorVβ

PnorV_beta is an optimized nitric oxide sensitive promoter. The promoter is coupled to a positive feedback loop with sfGFP and norR, the corresponding transcription factor. IHF was removed from the promoter PnorV to exhibit good sensitivity and dosage response at a low range of inducer DETA/NO, the used NO source in the experiment, activating the downstream genes' transcription.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]