Difference between revisions of "Part:BBa K4140000"
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==Usage== | ==Usage== | ||
| − | Because they are not recognized by E. coli RNAP, T7 promoters can generate considerable levels of transcription in E. coli and have the capacity to regulate themselves independently of E. coli promoters. We employ it to regulate TyrR protein expression constitutively in high | + | Because they are not recognized by E. coli RNAP, T7 promoters can generate considerable levels of transcription in E. coli and have the capacity to regulate themselves independently of E. coli promoters. We employ it to regulate TyrR protein expression constitutively in high levels |
==Literature Characterization== | ==Literature Characterization== | ||
Revision as of 17:36, 28 September 2022
T7 promoter
Part Description
T7 promoter is a dna sequence that can be recognized by T7 RNA polymerase and is mainly used to regulate gene expression of recombinant proteins,all of which allows it to be used in downstream research approaches.It is considered to be a constitutive promoter with high levels of transcription and expression in the presence of T7 RNAP and minimal interaction with bacterial RNAP.
Usage
Because they are not recognized by E. coli RNAP, T7 promoters can generate considerable levels of transcription in E. coli and have the capacity to regulate themselves independently of E. coli promoters. We employ it to regulate TyrR protein expression constitutively in high levels
Literature Characterization
References
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]