Difference between revisions of "Part:BBa K4140002"
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==Part Description==
 
==Part Description==
 
Sequence upstream of the start codon is crucial for the recruitment of a ribosome during the initiation of translation, which influences the rate and efficiency at which a certain Open Reading Frame (ORF) is translated by the following mechanisms:
 
Sequence upstream of the start codon is crucial for the recruitment of a ribosome during the initiation of translation, which influences the rate and efficiency at which a certain Open Reading Frame (ORF) is translated by the following mechanisms:
 +
<br>
 
-the rate of ribosomal recruitment to RBS
 
-the rate of ribosomal recruitment to RBS
 +
<br>
 
-the speed at which a recruited ribosome is able to start translation (i.e. the translation initiation efficiency)
 
-the speed at which a recruited ribosome is able to start translation (i.e. the translation initiation efficiency)
 +
<br>
 
-The ideal spacing of the RBS from the start codon
 
-The ideal spacing of the RBS from the start codon
  

Revision as of 16:46, 28 September 2022


TyrR RBS


Part Description

Sequence upstream of the start codon is crucial for the recruitment of a ribosome during the initiation of translation, which influences the rate and efficiency at which a certain Open Reading Frame (ORF) is translated by the following mechanisms:
-the rate of ribosomal recruitment to RBS
-the speed at which a recruited ribosome is able to start translation (i.e. the translation initiation efficiency)
-The ideal spacing of the RBS from the start codon

Usage

Literature Characterization

Figure 1.Substrate RNA cleavage assay using wild-type and mutant target RNAs.





























References

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]