Difference between revisions of "Part:BBa K4447001:Design"
Line 2: | Line 2: | ||
__NOTOC__ | __NOTOC__ | ||
<partinfo>BBa_K4447001 short</partinfo> | <partinfo>BBa_K4447001 short</partinfo> | ||
− | + | <div style="text-align:justify;"> | |
<partinfo>BBa_K4447001 SequenceAndFeatures</partinfo> | <partinfo>BBa_K4447001 SequenceAndFeatures</partinfo> | ||
Line 13: | Line 13: | ||
===Source=== | ===Source=== | ||
− | The original sequence was reported by Stassi et al. (1993). Sequence can be obtained through GenBank (L05776). | + | The original sequence was reported by Stassi <i>et al.</i> (1993). Sequence can be obtained through GenBank (L05776). |
===References=== | ===References=== | ||
+ | |||
+ | [1]. Stassi, D., Donadio, S., Staver, M. J., & Katz, L. (1993). Identification of a <i>Saccharopolyspora erythraea</i> gene required for the final hydroxylation step in erythromycin biosynthesis. <i>Journal of bacteriology, 175</i>(1), 182–189. https://doi.org/10.1128/jb.175.1.182-189.1993 |
Revision as of 16:45, 28 September 2022
EryK coding sequence
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 1197
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
A gly-gly-ser spacer, and a polyhistidine-tag were added before stop codon for protein purification. NcoI and XhoI restriction sites were added in 5' and 3' ends for protein overexpression in pBAD/Myc-His plasmids.
Source
The original sequence was reported by Stassi et al. (1993). Sequence can be obtained through GenBank (L05776).