Difference between revisions of "Part:BBa K4140005"
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<partinfo>BBa_K4140005 short</partinfo> | <partinfo>BBa_K4140005 short</partinfo> | ||
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| + | ==Part Description== | ||
A protein called L7Ae that binds to RNA inhibits the targeted transcript's ability to be translated. Transcriptional repressors take longer to suppress the intended construct than L7Ae that regulate gene expression at the translational level. As it specifically targets a sequence on the 5' end of the RNA termed the 2x-kturn. | A protein called L7Ae that binds to RNA inhibits the targeted transcript's ability to be translated. Transcriptional repressors take longer to suppress the intended construct than L7Ae that regulate gene expression at the translational level. As it specifically targets a sequence on the 5' end of the RNA termed the 2x-kturn. | ||
L7Ae can be used to build more complicated genetic circuits that are regulated at both the translational and transcriptional levels | L7Ae can be used to build more complicated genetic circuits that are regulated at both the translational and transcriptional levels | ||
| + | ==Usage== | ||
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| + | ==Literature Characterization== | ||
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| + | [[File:T--AFCM-EGYPT--CAS2.PNG|thumb|Right|Figure 1.Substrate RNA cleavage assay using wild-type and mutant target RNAs. ]] | ||
| + | <br><br><br><br><br><br><br><br><br><br><br><br><br><br><br><br><br><br><br><br><br><br><br><br><br><br><br> | ||
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| + | ==References== | ||
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Revision as of 09:51, 28 September 2022
L7AE
Part Description
A protein called L7Ae that binds to RNA inhibits the targeted transcript's ability to be translated. Transcriptional repressors take longer to suppress the intended construct than L7Ae that regulate gene expression at the translational level. As it specifically targets a sequence on the 5' end of the RNA termed the 2x-kturn. L7Ae can be used to build more complicated genetic circuits that are regulated at both the translational and transcriptional levels
Usage
Literature Characterization
References
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]