Difference between revisions of "Part:BBa K4409010"

 
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This is an RNA-interacting guide RNA (igRNA). It is used in a CRISPR-Cas12 system. It mainly comprises the following three parts. The first part (BBa_K2965036) is the Cas12 handle which combines with Cas12 protein. The second part (BBA_K4409000) is a guide sequence targeting a DNA sequence of GAPDH. The last part (BBa_K4409004) is a control sequence made up of VR1, loop and VR2 regions in sequence. VR1 and VR2 regions can specifically bind to the RNA trigger which is a unique sequence of hsa_circ_0001982. The loop binds to a portion of the guide sequence.  
 
This is an RNA-interacting guide RNA (igRNA). It is used in a CRISPR-Cas12 system. It mainly comprises the following three parts. The first part (BBa_K2965036) is the Cas12 handle which combines with Cas12 protein. The second part (BBA_K4409000) is a guide sequence targeting a DNA sequence of GAPDH. The last part (BBa_K4409004) is a control sequence made up of VR1, loop and VR2 regions in sequence. VR1 and VR2 regions can specifically bind to the RNA trigger which is a unique sequence of hsa_circ_0001982. The loop binds to a portion of the guide sequence.  
 
When hsa_circ_0001982 is present in the CRISPR-Cas12 system, the control sequence binds with it and the igRNA is activated. The loop then unblocks the guide. The guide binds to a GAPDH dsDNA with the PAM sequence, and activates the Cas12 protein, resulting in the cleavage of dsDNA and ssDNA in the system.
 
When hsa_circ_0001982 is present in the CRISPR-Cas12 system, the control sequence binds with it and the igRNA is activated. The loop then unblocks the guide. The guide binds to a GAPDH dsDNA with the PAM sequence, and activates the Cas12 protein, resulting in the cleavage of dsDNA and ssDNA in the system.
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[[File:Hz1-part-1-3.png|500px|thumb|center|Graph 1 Figure Schematic representation of the inactive (A) and active(B,C) igRNA in a CRISPR-Cas12 system.]]
  
 
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Revision as of 05:11, 28 September 2022


igRNA (CT+GAPDH as guide+ hsa_circ_0001982 as trigger)

This is an RNA-interacting guide RNA (igRNA). It is used in a CRISPR-Cas12 system. It mainly comprises the following three parts. The first part (BBa_K2965036) is the Cas12 handle which combines with Cas12 protein. The second part (BBA_K4409000) is a guide sequence targeting a DNA sequence of GAPDH. The last part (BBa_K4409004) is a control sequence made up of VR1, loop and VR2 regions in sequence. VR1 and VR2 regions can specifically bind to the RNA trigger which is a unique sequence of hsa_circ_0001982. The loop binds to a portion of the guide sequence. When hsa_circ_0001982 is present in the CRISPR-Cas12 system, the control sequence binds with it and the igRNA is activated. The loop then unblocks the guide. The guide binds to a GAPDH dsDNA with the PAM sequence, and activates the Cas12 protein, resulting in the cleavage of dsDNA and ssDNA in the system.

Graph 1 Figure Schematic representation of the inactive (A) and active(B,C) igRNA in a CRISPR-Cas12 system.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]