Difference between revisions of "Part:BBa K4232000"

Revision as of 02:21, 28 September 2022

It is a widely regarded catalyst that oxidizes many substrates and reduces oxygen to water.

This part of the code is laccase. Laccase, known as multicopper oxidase CueO, is found in Escherichia coli. It is a multinucleated copper-containing oxidase widely found in plants, animals and microorganisms that oxidises many reducing substrates while reducing oxygen to water, and can therefore act as an oxygen consumer (the specific mechanism of action is shown in Figure 1).

In using laccase it is necessary to consider whether to introduce a signal peptide. cueO has 516 amino acid residues, but the first 28 amino acids are signal peptides that will localise the laccase to the periplasm. If it is desired for CueO to function within the cell, the sequence corresponding to the signal peptide can be removed.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 225
1000
COMPATIBLE WITH RFC[1000]

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 <partinfo>BBa_K4232000 short</partinfo>
-This part of the code is laccase. full name multicopper oxidase CueO, is derived from E. coli. It is a multinuclear copper-containing oxidase widely found in plants and animals, microorganisms, can oxidize many reducing substrates while reducing oxygen to water, so it can play the role of oxygen consumption.
+[[File:File.png|400px|thumb|left]]
+This part of the code is laccase. Laccase, known as multicopper oxidase '''CueO''', is found in Escherichia coli. It is a multinucleated copper-containing oxidase widely found in plants, animals and microorganisms that oxidises many reducing substrates while reducing oxygen to water, and can therefore act as an oxygen consumer (the specific mechanism of action is shown in Figure 1).
+In using laccase it is necessary to consider whether to introduce a signal peptide. cueO has 516 amino acid residues, but the first 28 amino acids are signal peptides that will localise the laccase to the periplasm. If it is desired for CueO to function within the cell, the sequence corresponding to the signal peptide can be removed.
-The introduction of a signal peptide needs to be considered in the use of laccase. cueO has 516 amino acid residues, but the first 28 amino acids are signal peptides that will localize laccase to the periplasm. If CueO is desired to function intracellularly, the sequence corresponding to the signal peptide can be removed.
-Reference：Modulation of Intracellular O2 Concentration in Escherichia coli Strains Using Oxygen Consuming Devices（ACS Synth. Biol. 2018, 7, 1742−1752）