Difference between revisions of "Part:BBa K4324001:Design"

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LacPO:  
 
LacPO:  
  
−
- RBS:  
+
RBS:  
  
−
- CDS: XYL2 gene from Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast). Taken from the [https://www.uniprot.org/uniprotkb/Q07993/entry Uniprot database].
+
CDS: XYL2 gene from Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast). Taken from the [https://www.uniprot.org/uniprotkb/Q07993/entry Uniprot database].
  
−
- Terminator:
+
Terminator:
  
 
===References===
 
===References===

Revision as of 01:28, 28 September 2022


lacPO-RBS-XDH-T1


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 700
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 357


Design Notes

The protein sequence was codon-optimised for Escherichia coli through the IDT codon optimisation tool as gBlocks Gene Fragments.



Source

LacPO:

RBS:

CDS: XYL2 gene from Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast). Taken from the Uniprot database.

Terminator:

References