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<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 23:57, 27 September 2022


PT7_obcA

1


Contribution From CAU_China 2022

Group: CAU_China,2022 https://2022.igem.org/Team:CAU_China

Author: Liu Muhua

Summary: Verified that this composite Part can produce oxalic acid in Escherichia coli

Characterization

CAU_China 2022 used the CDS sequence in their oxalate secretion pathway and the expression product of obcA is one of the main enzyme in this pathway. It canact together with ObcB to catalyze oxaloacetic acid (OAA) and citric acid to form oxalic acid (OA). When there is only ObcA, it has a low ability to form oxalic acidin our project.

Hence, we aim to verify that the gene obcA, originally from Burkholderia glumae, can be regulated by PT7 and lac operator and properly expressed downstream to produce oxalic acid in Escherichia coli.



Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 276
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 276
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1420
    Illegal XhoI site found at 183
    Illegal XhoI site found at 1307
    Illegal XhoI site found at 1751
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 276
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 276
    Illegal NgoMIV site found at 268
    Illegal NgoMIV site found at 599
    Illegal NgoMIV site found at 627
    Illegal NgoMIV site found at 663
    Illegal NgoMIV site found at 893
    Illegal NgoMIV site found at 1670
    Illegal NgoMIV site found at 1721
    Illegal AgeI site found at 205
  • 1000
    COMPATIBLE WITH RFC[1000]