Difference between revisions of "Part:BBa K4447002:Design"
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===Design Notes===
 
===Design Notes===
 
A gly-gly-ser spacer, and a polyhistidine-tag were added before stop codon for protein purification. NcoI and XhoI restriction sites were added in 5' and 3' ends for protein overexpression in pBAD/Myc-His plasmids.
 
A gly-gly-ser spacer, and a polyhistidine-tag were added before stop codon for protein purification. NcoI and XhoI restriction sites were added in 5' and 3' ends for protein overexpression in pBAD/Myc-His plasmids.
 +
  
 
===Source===
 
===Source===
  
 
The original sequence was reported by Orser et al. (1993). Sequence can be obtained through GenBank (M98557.1).
 
The original sequence was reported by Orser et al. (1993). Sequence can be obtained through GenBank (M98557.1).
 +
  
 
===References===
 
===References===
  
 
[1]. Orser, C. S., Lange, C. C., Xun, L., Zahrt, T. C., & Schneider, B. J. (1993). Cloning, sequence analysis, and expression of the Flavobacterium pentachlorophenol-4-monooxygenase gene in <i>Escherichia coli</i>. <i>Journal of bacteriology, 175</i>(2), 411–416. https://doi.org/10.1128/jb.175.2.411-416.1993
 
[1]. Orser, C. S., Lange, C. C., Xun, L., Zahrt, T. C., & Schneider, B. J. (1993). Cloning, sequence analysis, and expression of the Flavobacterium pentachlorophenol-4-monooxygenase gene in <i>Escherichia coli</i>. <i>Journal of bacteriology, 175</i>(2), 411–416. https://doi.org/10.1128/jb.175.2.411-416.1993

Revision as of 16:04, 27 September 2022


Pcp coding sequence


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 1651
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

Design Notes

A gly-gly-ser spacer, and a polyhistidine-tag were added before stop codon for protein purification. NcoI and XhoI restriction sites were added in 5' and 3' ends for protein overexpression in pBAD/Myc-His plasmids.


Source

The original sequence was reported by Orser et al. (1993). Sequence can be obtained through GenBank (M98557.1).


References

[1]. Orser, C. S., Lange, C. C., Xun, L., Zahrt, T. C., & Schneider, B. J. (1993). Cloning, sequence analysis, and expression of the Flavobacterium pentachlorophenol-4-monooxygenase gene in Escherichia coli. Journal of bacteriology, 175(2), 411–416. https://doi.org/10.1128/jb.175.2.411-416.1993