Difference between revisions of "Part:BBa K4256005"
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<partinfo>BBa_K4256005 short</partinfo> | <partinfo>BBa_K4256005 short</partinfo> | ||
| − | The original sequence is from Arabidopsis thaliana (thale cress), encoding O-methyltransferase 1. Caffeate O-methyltransferase can convert caffeic acid to ferulic acid. To synthesize this enzyme in E. coli, the sequence was codon-optimized by GenSmart. Reference: Tabata S, Kaneko T, Nakamura Y, Kotani H, Kato T, etc. Sequence and analysis of chromosome 5 of the plant Arabidopsis thaliana. Nature. 2000 Dec 14;408(6814):823-6. | + | The original sequence is from <i>Arabidopsis thaliana</i> (thale cress), encoding O-methyltransferase 1. Caffeate O-methyltransferase can convert caffeic acid to ferulic acid. To synthesize this enzyme in <i>E. coli</i>, the sequence was codon-optimized by GenSmart. Reference: Tabata S, Kaneko T, Nakamura Y, Kotani H, Kato T, etc. Sequence and analysis of chromosome 5 of the plant Arabidopsis thaliana.<i> Nature.</i> 2000 Dec 14;408(6814):823-6. |
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Latest revision as of 15:10, 27 September 2022
comt (caffeate O-methyltransferase) from A. thaliana (optimized)
The original sequence is from Arabidopsis thaliana (thale cress), encoding O-methyltransferase 1. Caffeate O-methyltransferase can convert caffeic acid to ferulic acid. To synthesize this enzyme in E. coli, the sequence was codon-optimized by GenSmart. Reference: Tabata S, Kaneko T, Nakamura Y, Kotani H, Kato T, etc. Sequence and analysis of chromosome 5 of the plant Arabidopsis thaliana. Nature. 2000 Dec 14;408(6814):823-6.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 563
Illegal BamHI site found at 498 - 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]