Difference between revisions of "Part:BBa K4235029"
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| − | This part is the entire circuit of the Ampicillin Resistance gene which is used within the plasmid and it confers resistance to Ampicillin. The expression of the ampicillin coding sequence is driven by the AmpR promoter <partinfo>BBa_K4235017</partinfo>. This marker was used for the selection of successful transformants after cloning our gene insert <partinfo>BBa_K4235011</partinfo> in the plasmid. | + | This part is the entire circuit of the Ampicillin Resistance gene <partinfo>BBa_K4235018</partinfo> which is used within the plasmid and it confers resistance to Ampicillin. The expression of the ampicillin coding sequence is driven by the AmpR promoter <partinfo>BBa_K4235017</partinfo>. This marker was used for the selection of successful transformants after cloning our gene insert <partinfo>BBa_K4235011</partinfo> in the plasmid. |
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Latest revision as of 14:55, 27 September 2022
Ampicillin resistance gene cassette
Usage and Biology
This part is the entire circuit of the Ampicillin Resistance gene BBa_K4235018 which is used within the plasmid and it confers resistance to Ampicillin. The expression of the ampicillin coding sequence is driven by the AmpR promoter BBa_K4235017. This marker was used for the selection of successful transformants after cloning our gene insert BBa_K4235011 in the plasmid.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 647
- 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 647
- 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 647
- 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 647
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 821