Difference between revisions of "Part:BBa K4235025"

(→‎Usage and Biology)
(→‎Usage and Biology)
 
Line 7: Line 7:
  
 
===Usage and Biology===
 
===Usage and Biology===
−
This part is the entire circuit of the Ampicillin Resistance gene which is used within the plasmid <partinfo>BBa_K4235002</partinfo> and it confers resistance to Ampicillin. The expression of the ampicillin coding sequence is driven by the AmpR promoter <partinfo>BBa_K4235006</partinfo>. This marker was used for the selection of successful transformants after cloning our gene insert <partinfo>BBa_K4235000</partinfo> in the plasmid pFastBac.  
+
This part is the entire circuit of the Ampicillin Resistance gene which is used within the plasmid <partinfo>BBa_K4235002</partinfo> and it confers resistance to Ampicillin. The expression of the ampicillin coding sequence is driven by the AmpR promoter <partinfo>BBa_K2308010</partinfo>. This marker was used for the selection of successful transformants after cloning our gene insert <partinfo>BBa_K4235000</partinfo> in the plasmid pFastBac.  
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Latest revision as of 14:48, 27 September 2022


Ampicillin resistance gene cassette

Introduction Ampicillin is one of the most common selection markers used in synthetic biology. Its structure contains a β-lactam ring that inhibits proteins involved in bacterial peptidoglycan synthesis. As a result, bacteria cannot synthesize new peptidoglycan which disrupts the formation of cell walls and leads to cell death. The ampicillin resistance gene encodes for the enzyme β-lactamase, which cleaves the β-lactam ring and inactivates the ampicillin, thereby providing resistance to the bacteria.

Usage and Biology

This part is the entire circuit of the Ampicillin Resistance gene which is used within the plasmid BBa_K4235002 and it confers resistance to Ampicillin. The expression of the ampicillin coding sequence is driven by the AmpR promoter BBa_K2308010. This marker was used for the selection of successful transformants after cloning our gene insert BBa_K4235000 in the plasmid pFastBac.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 821