Difference between revisions of "Part:BBa K4235032"
| Line 3: | Line 3: | ||
<partinfo>BBa_K4235032 short</partinfo> | <partinfo>BBa_K4235032 short</partinfo> | ||
| − | This is the primer sequence used to amplify the insert <partinfo>BBa_K4235000</partinfo> and create 3' overhangs for the consecutive LIC reaction. | + | This is the reverse primer sequence used to amplify the insert <partinfo>BBa_K4235000</partinfo> and create 3' overhangs for the consecutive LIC reaction. |
<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here | ||
Latest revision as of 14:42, 27 September 2022
LIC Primer (insert 3')
This is the reverse primer sequence used to amplify the insert BBa_K4235000 and create 3' overhangs for the consecutive LIC reaction.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 16
- 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 16
- 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 16
- 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 16
- 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 16
- 1000COMPATIBLE WITH RFC[1000]