Difference between revisions of "Part:BBa K190016:Design"
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===Design Notes=== | ===Design Notes=== | ||
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| + | The ZntR regulated promoter has been cloned into the [https://parts.igem.org/Part:BBa_J61002 BBa_J61002-R0040] plasmid for construction of promoter basic parts and their derivatives. | ||
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| + | Insertion of a promoter element between the XbaI and SpeI sites resulted in a RFP reporter while retaining the ability to do biobrick assembly. | ||
| + | [[Image:PBca1020-r0040.jpg|200px]] | ||
===Source=== | ===Source=== | ||
Revision as of 12:37, 13 August 2009
Zinc Promoter (ZntR regulated)
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
The ZntR regulated promoter has been cloned into the BBa_J61002-R0040 plasmid for construction of promoter basic parts and their derivatives.
Insertion of a promoter element between the XbaI and SpeI sites resulted in a RFP reporter while retaining the ability to do biobrick assembly.
Source
E.coli TOP10