Difference between revisions of "Part:BBa K176054:Design"

 
 
Line 1: Line 1:
−
 
 
__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K176054 short</partinfo>
 
<partinfo>BBa_K176054 short</partinfo>
Line 9: Line 8:
 
Designed with our software tool, which is modified from primer3 to pick primers from random sequences for synthetic biological primer design.
 
Designed with our software tool, which is modified from primer3 to pick primers from random sequences for synthetic biological primer design.
  
 +
Main parameters for picking VR3~VR12:
 +
 +
Size: 20 bp (N19 + G or C)
 +
 +
Tm: VF2 Tm ±1℃
 +
 +
GC%: 50%
 +
 +
Max Self Complementarity: 3 bp
 +
 +
Max 3' Self Complementarity: 2 bp
 +
 +
Max Pair Complementarity: 3 bp
 +
 +
Max 3' Pair Complementarity: 2 bp
 +
 +
Max 3' Stability: 3.36 kcal/mol
 +
 +
Max Template(pSB1A3) Mispriming: 6 bp
  
 +
Max Mispriming With Other VR3~VR12 Sites: 4 bp
  
 
===Source===
 
===Source===

Latest revision as of 09:26, 13 August 2009

Reverse primer VR10 (bind to K176044), member of VR3~VR12


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Designed with our software tool, which is modified from primer3 to pick primers from random sequences for synthetic biological primer design.

Main parameters for picking VR3~VR12:

Size: 20 bp (N19 + G or C)

Tm: VF2 Tm ±1℃

GC%: 50%

Max Self Complementarity: 3 bp

Max 3' Self Complementarity: 2 bp

Max Pair Complementarity: 3 bp

Max 3' Pair Complementarity: 2 bp

Max 3' Stability: 3.36 kcal/mol

Max Template(pSB1A3) Mispriming: 6 bp

Max Mispriming With Other VR3~VR12 Sites: 4 bp

Source

Reverse complement of K176044. Picked from random sequences.

References