Difference between revisions of "Part:BBa K4329003"

Revision as of 12:04, 27 September 2022

Quorum sensing suicidal system-part1

what is it

The quorum sensing suicidal system consists of three parts: LUXR, CRISPR/Cas9, sgRNA. The LUXR protein is regulated by T7 promoter, lacI operator, and T7 terminator. CRISPR/Cas9 is regulated by a quorum sensing promoter and quorum sensing terminator. sgRNA is regulated by H1 promoter and H1 terminator.

what it does

At first, if the IPTG is absent, the RNA ligase in the system will converge with T7 promoter which can not lead to the production of mRNA. Thus, protein and enzyme production will be inhibited. However, in severe situations, IPTG could be present in the natural environment, so we have considered a double safety system. If LUXR detects the presence of AHL, a quorum sensing chemical sent out by E.Coli, quorum sensing promoter will be activated. Later, the promoter will activate the CRISPRi system targeting the ATP ligase of E.coli. Consequently, without ATP, the bacteria will be forced to die.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

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 ===what it does===
+At first, if the IPTG is absent, the RNA ligase in the system will converge with T7 promoter which can not lead to the production of mRNA. Thus, protein and enzyme production will be inhibited. However, in severe situations, IPTG could be present in the natural environment, so we have considered a double safety system. If LUXR detects the presence of AHL, a quorum sensing chemical sent out by E.Coli, quorum sensing promoter will be activated. Later, the promoter will activate the CRISPRi system targeting the ATP ligase of E.coli. Consequently, without ATP, the bacteria will be forced to die.
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