Difference between revisions of "Part:BBa K4207002"
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Toehold switches are de novo designed riboregulators that can be used to sense different nucleic acid sequences. They are specifically designed RNA sequences that have the ribosome binding site (RBS) and the start codon in a stem-loop followed by a reporter gene. The RBS and the start codon are sequestered in the secondary structure, which hinder the translation of the reporter gene. The toehold switch has a specific binding site to its trigger sequence, which extends to the base of the stem-loop. When the trigger binds, it unwinds the lower part of the stem-loop, leaving only a weak secondary structure intact. This remaining structure is designed to be weak, so ribosome binding unwinds the structure, allowing translation to occur. (Green et. al., 2014) (Green et. al., 2017). | Toehold switches are de novo designed riboregulators that can be used to sense different nucleic acid sequences. They are specifically designed RNA sequences that have the ribosome binding site (RBS) and the start codon in a stem-loop followed by a reporter gene. The RBS and the start codon are sequestered in the secondary structure, which hinder the translation of the reporter gene. The toehold switch has a specific binding site to its trigger sequence, which extends to the base of the stem-loop. When the trigger binds, it unwinds the lower part of the stem-loop, leaving only a weak secondary structure intact. This remaining structure is designed to be weak, so ribosome binding unwinds the structure, allowing translation to occur. (Green et. al., 2014) (Green et. al., 2017). | ||
| − | Here we designed a A-series toehold switch, which has a structure allowing lower translational leakage to previous toehold switches (Pardee et. al., 2016). This toehold switch is designed to detect the presence of barley yellow dwarf virus gRNA by binding to a conserved sequence found in the virus' | + | Here we designed a A-series toehold switch, which has a structure allowing lower translational leakage to previous toehold switches (Pardee et. al., 2016). This toehold switch is designed to detect the presence of barley yellow dwarf virus gRNA by binding to a conserved sequence found in the virus' enome. |
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Revision as of 10:52, 27 September 2022
BYDV toehold switch A70
Toehold switch for the detection of BYDV gRNA
Usage and Biology
Toehold switches are de novo designed riboregulators that can be used to sense different nucleic acid sequences. They are specifically designed RNA sequences that have the ribosome binding site (RBS) and the start codon in a stem-loop followed by a reporter gene. The RBS and the start codon are sequestered in the secondary structure, which hinder the translation of the reporter gene. The toehold switch has a specific binding site to its trigger sequence, which extends to the base of the stem-loop. When the trigger binds, it unwinds the lower part of the stem-loop, leaving only a weak secondary structure intact. This remaining structure is designed to be weak, so ribosome binding unwinds the structure, allowing translation to occur. (Green et. al., 2014) (Green et. al., 2017).
Here we designed a A-series toehold switch, which has a structure allowing lower translational leakage to previous toehold switches (Pardee et. al., 2016). This toehold switch is designed to detect the presence of barley yellow dwarf virus gRNA by binding to a conserved sequence found in the virus' enome.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]