Difference between revisions of "Part:BBa K176047:Design"
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<partinfo>BBa_K176047 short</partinfo> | <partinfo>BBa_K176047 short</partinfo> | ||
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Designed with our software tool, which is modified from primer3 to pick primers from random sequences for synthetic biological primer design. | Designed with our software tool, which is modified from primer3 to pick primers from random sequences for synthetic biological primer design. | ||
+ | Main parameters for picking VR3~VR12: | ||
+ | |||
+ | Size: 20 bp (N19 + G or C) | ||
+ | |||
+ | Tm: VF2 Tm ±1℃ | ||
+ | |||
+ | GC%: 50% | ||
+ | |||
+ | Max Self Complementarity: 3 bp | ||
+ | |||
+ | Max 3' Self Complementarity: 2 bp | ||
+ | |||
+ | Max Pair Complementarity: 3 bp | ||
+ | |||
+ | Max 3' Pair Complementarity: 2 bp | ||
+ | |||
+ | Max 3' Stability: 3.36 kcal/mol | ||
+ | |||
+ | Max Template(pSB1A3) Mispriming: 6 bp | ||
+ | Max Mispriming With Other VR3~VR12 Sites: 4 bp | ||
===Source=== | ===Source=== |
Latest revision as of 09:23, 13 August 2009
Reverse primer VR3 (bind to K176037), member of VR3~VR12
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Designed with our software tool, which is modified from primer3 to pick primers from random sequences for synthetic biological primer design.
Main parameters for picking VR3~VR12:
Size: 20 bp (N19 + G or C)
Tm: VF2 Tm ±1℃
GC%: 50%
Max Self Complementarity: 3 bp
Max 3' Self Complementarity: 2 bp
Max Pair Complementarity: 3 bp
Max 3' Pair Complementarity: 2 bp
Max 3' Stability: 3.36 kcal/mol
Max Template(pSB1A3) Mispriming: 6 bp
Max Mispriming With Other VR3~VR12 Sites: 4 bp
Source
Reverse complement of K176037. Picked from random sequences.