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This mutant contains no nucleotide substitutions or indels outside of the targeted site.
 
This mutant contains no nucleotide substitutions or indels outside of the targeted site.
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Revision as of 09:22, 27 September 2022


BlcR V68T

A mutant of the BlcR protein, created through site-directed mutagenesis with primers R3 (Part:BBa_K4361218) and F3.7 V68T (Part:BBa_K4361225). For this mutant, the valine in position 68 has been changed to threonine by mutating the GTG codon to ACC.

This mutant contains no nucleotide substitutions or indels outside of the targeted site.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 694
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 78
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 589