Difference between revisions of "Part:BBa K4409003"
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It is used as a sensing part of the RNA-interacting guide RNA (igRNA) in our CRISPR-Cas12 system to detect a unique sequence (trigger RNA) of the target circRNA hsa_circ_0001785. This part serves as a switch for the whole circuit. It contains three regions, VR1 region, loop region, and VR2 region. VR1 and VR2 regions complement to VR1’ and VR2’ regions (trigger RNA) of hsa_circ_0001785. Loop region is complementary to the guide of igRNA (hsa_circ_0001982) (BBa_K4409002). When hsa_circ_0001785 is present in the system, VR1 and VR2 regions combine with VR1’ and VR2’ sequences. Thus, loop region unblocks the guide of igRNA (BBa_K4409002) which can then bind with dsDNA(hsa_circ_0001982) (BBa_K4409008) and activate Cas enzyme to cleave DNAs. | It is used as a sensing part of the RNA-interacting guide RNA (igRNA) in our CRISPR-Cas12 system to detect a unique sequence (trigger RNA) of the target circRNA hsa_circ_0001785. This part serves as a switch for the whole circuit. It contains three regions, VR1 region, loop region, and VR2 region. VR1 and VR2 regions complement to VR1’ and VR2’ regions (trigger RNA) of hsa_circ_0001785. Loop region is complementary to the guide of igRNA (hsa_circ_0001982) (BBa_K4409002). When hsa_circ_0001785 is present in the system, VR1 and VR2 regions combine with VR1’ and VR2’ sequences. Thus, loop region unblocks the guide of igRNA (BBa_K4409002) which can then bind with dsDNA(hsa_circ_0001982) (BBa_K4409008) and activate Cas enzyme to cleave DNAs. | ||
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| + | <div style='text-align: center;'> | ||
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| + | [[File:Hz1-part-1-3.png|600px|thumb|center]] | ||
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| + | <b> 【Graph 1】The made up of igRNA </b> | ||
| + | </div> | ||
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Latest revision as of 08:04, 27 September 2022
trigger-RNA sensor of igRNA (hsa_circ_0001785)
It is used as a sensing part of the RNA-interacting guide RNA (igRNA) in our CRISPR-Cas12 system to detect a unique sequence (trigger RNA) of the target circRNA hsa_circ_0001785. This part serves as a switch for the whole circuit. It contains three regions, VR1 region, loop region, and VR2 region. VR1 and VR2 regions complement to VR1’ and VR2’ regions (trigger RNA) of hsa_circ_0001785. Loop region is complementary to the guide of igRNA (hsa_circ_0001982) (BBa_K4409002). When hsa_circ_0001785 is present in the system, VR1 and VR2 regions combine with VR1’ and VR2’ sequences. Thus, loop region unblocks the guide of igRNA (BBa_K4409002) which can then bind with dsDNA(hsa_circ_0001982) (BBa_K4409008) and activate Cas enzyme to cleave DNAs.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]