Difference between revisions of "Part:BBa K4409000:Design"
 
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This part with CT sequence is designed based on the BBa_K2961003 part from GreatBay_SZ team. The aim for this modification is to increase the sensitivity of this handle region by enhancing the ability for Cas12a to combine with gRNA. Thus, we change three base pairs from AAG to CT.  
 
This part with CT sequence is designed based on the BBa_K2961003 part from GreatBay_SZ team. The aim for this modification is to increase the sensitivity of this handle region by enhancing the ability for Cas12a to combine with gRNA. Thus, we change three base pairs from AAG to CT.  
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[[File:Hz1-part-1-1.png|600px|thumb|center]]
 
[[File:Hz1-part-1-1.png|600px|thumb|center]]
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<b> 【Graph 1】The difference between this part and BBa_K2961003 </b>
 
<b> 【Graph 1】The difference between this part and BBa_K2961003 </b>
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We incorporated this part into the composite parts to construct igRNA (BBa_K4409007) and tested its sensitivity with the igRNA containing BBa_K2961003 (BBa_K4409010). No significant diference was observed in the sensitivy between the two parts. This part is equally suited for Cas12a handle region as BBa_K2961003.  
 
We incorporated this part into the composite parts to construct igRNA (BBa_K4409007) and tested its sensitivity with the igRNA containing BBa_K2961003 (BBa_K4409010). No significant diference was observed in the sensitivy between the two parts. This part is equally suited for Cas12a handle region as BBa_K2961003.  

Latest revision as of 07:57, 27 September 2022


gRNA / crRNA fixed-loop repeat for Cas12a


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part with CT sequence is designed based on the BBa_K2961003 part from GreatBay_SZ team. The aim for this modification is to increase the sensitivity of this handle region by enhancing the ability for Cas12a to combine with gRNA. Thus, we change three base pairs from AAG to CT.

Hz1-part-1-1.png
Hz1-part-1-2.png

【Graph 1】The difference between this part and BBa_K2961003

We incorporated this part into the composite parts to construct igRNA (BBa_K4409007) and tested its sensitivity with the igRNA containing BBa_K2961003 (BBa_K4409010). No significant diference was observed in the sensitivy between the two parts. This part is equally suited for Cas12a handle region as BBa_K2961003.

Source

This part is based on BBa_K2961003 with a slight modification.

References