Difference between revisions of "Part:BBa K4409005"

 
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<partinfo>BBa_K4409005 short</partinfo>
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It is used as a sensing part of the RNA-interacting guide RNA (igRNA) in our CRISPR-Cas12 system to detect a unique sequence (trigger RNA) of the target circRNA hsa_circ_0001982. This part serves as a switch for the whole circuit. It contains three regions, VR1 region, loop region, and VR2 region. VR1 and VR2 regions complement to VR1’ and VR2’ regions (trigger RNA) of hsa_circ_0001982. Loop region is complementary to the guide of igRNA (hsa_circ_0001785) (BBa_K4409001). When hsa_circ_0001982 is present in the system, VR1 and VR2 regions combine with VR1’ and VR2’ sequences. Thus, loop region unblocks the guide of igRNA (hsa_circ_0001785) (BBa_K4409001) which can then bind with dsDNA(hsa_circ_0001785) (BBa_K4409007) and activate Cas enzyme to cleave DNAs.
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===Usage and Biology===
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<span class='h3bb'>Sequence and Features</span>
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<partinfo>BBa_K4409005 SequenceAndFeatures</partinfo>
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===Functional Parameters===
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<partinfo>BBa_K4409005 parameters</partinfo>
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Revision as of 07:27, 27 September 2022


trigger-RNA sensor of igRNA (hsa_circ_0001982)_coupled with hsa_circ_0001785 as the guide sequence

It is used as a sensing part of the RNA-interacting guide RNA (igRNA) in our CRISPR-Cas12 system to detect a unique sequence (trigger RNA) of the target circRNA hsa_circ_0001982. This part serves as a switch for the whole circuit. It contains three regions, VR1 region, loop region, and VR2 region. VR1 and VR2 regions complement to VR1’ and VR2’ regions (trigger RNA) of hsa_circ_0001982. Loop region is complementary to the guide of igRNA (hsa_circ_0001785) (BBa_K4409001). When hsa_circ_0001982 is present in the system, VR1 and VR2 regions combine with VR1’ and VR2’ sequences. Thus, loop region unblocks the guide of igRNA (hsa_circ_0001785) (BBa_K4409001) which can then bind with dsDNA(hsa_circ_0001785) (BBa_K4409007) and activate Cas enzyme to cleave DNAs.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]