Difference between revisions of "Part:BBa K4409004"

Revision as of 07:25, 27 September 2022

trigger-RNA sensor of igRNA (hsa_circ_0001982)_Coupled with GAPDH as guide sequence

It is used as a sensing part of the RNA-interacting guide RNA (igRNA) in our CRISPR-Cas12 system to detect a unique sequence (trigger RNA) of the target circRNA hsa_circ_0001982. This part serves as a switch for the whole circuit. It contains three regions, VR1 region, loop region, and VR2 region. VR1 and VR2 regions complement to VR1’ and VR2’ regions (trigger RNA) of hsa_circ_0001982. Loop region is complementary to the guide of igRNA (GAPDH) (BBa_K4409017). When hsa_circ_0001982 is present in the system, VR1 and VR2 regions combine with VR1’ and VR2’ sequences. Thus, loop region unblocks the guide of igRNA (GAPDH) (BBa_K4409017) which can then bind with dsDNA (GAPDH) (BBa_K4409006) and activate Cas enzyme to cleave DNAs.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

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 <partinfo>BBa_K4409004 short</partinfo>
+It is used as a sensing part of the RNA-interacting guide RNA (igRNA) in our CRISPR-Cas12 system to detect a unique sequence (trigger RNA) of the target circRNA hsa_circ_0001982. This part serves as a switch for the whole circuit. It contains three regions, VR1 region, loop region, and VR2 region. VR1 and VR2 regions complement to VR1’ and VR2’ regions (trigger RNA) of hsa_circ_0001982. Loop region is complementary to the guide of igRNA (GAPDH) (BBa_K4409017). When hsa_circ_0001982 is present in the system, VR1 and VR2 regions combine with VR1’ and VR2’ sequences. Thus, loop region unblocks the guide of igRNA (GAPDH) (BBa_K4409017) which can then bind with dsDNA (GAPDH) (BBa_K4409006) and activate Cas enzyme to cleave DNAs.
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