Difference between revisions of "Part:BBa K4046050"
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This is the Kozak sequence used by the 2021 Duke iGEM team for constructing our plasmids. The Kozak sequence is meant to facilitate the proper initiation of translation in eukaryotic mRNA transcripts. The Kozak sequence is inserted immediately upstream of our reporter protein within each construct.  
 
This is the Kozak sequence used by the 2021 Duke iGEM team for constructing our plasmids. The Kozak sequence is meant to facilitate the proper initiation of translation in eukaryotic mRNA transcripts. The Kozak sequence is inserted immediately upstream of our reporter protein within each construct.  
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===Part Contributions===
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'''Group:''' iGEM22_Stony_Brook
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'''Authors:''' Maulik Masaliya
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'''Contribution Summary'''<br>
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This is the vertebrate consensus Kozak sequence, added upstream of the start codon of our insert
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<partinfo>BBa_K4235000</partinfo>. The Kozak sequence serves as a translation initiation site in eukaryotes and ensures proper translation the protein from the mRNA transcript.
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This marker is used to provide gentamicin resistance to the pFastBac vector during the transposition in the Bacmid, propagated in E coli DH10Bac cells. A concentration of 7 ug/ml gentamicin is recommended for transposition.
  
 
<!-- Uncomment this to enable Functional Parameter display  
 
<!-- Uncomment this to enable Functional Parameter display  

Revision as of 01:17, 27 September 2022


Kozak Sequence (Human RBS)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

This is the Kozak sequence used by the 2021 Duke iGEM team for constructing our plasmids. The Kozak sequence is meant to facilitate the proper initiation of translation in eukaryotic mRNA transcripts. The Kozak sequence is inserted immediately upstream of our reporter protein within each construct.

Part Contributions

Group: iGEM22_Stony_Brook

Authors: Maulik Masaliya

Contribution Summary
This is the vertebrate consensus Kozak sequence, added upstream of the start codon of our insert BBa_K4235000. The Kozak sequence serves as a translation initiation site in eukaryotes and ensures proper translation the protein from the mRNA transcript.

This marker is used to provide gentamicin resistance to the pFastBac vector during the transposition in the Bacmid, propagated in E coli DH10Bac cells. A concentration of 7 ug/ml gentamicin is recommended for transposition.